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KMID : 0350519940470041761
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1761 ~ p.1775
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Effect of Hyaluronic Acid on Corneal Re-epithelization of Rat following Excimer Laser Keratectomy
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Abstract
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The effect of hyaluronic acid(HA) on corneal re-epithelization following excimer laser keratectomy was evaluated in rats. An argon fluoride excimer laser(193 nm) with a moving slit delivery system was used to perform laser keratectomy in each eye
of 44
rats(4 mm in diameter and 38μm in depth). The animals were divided into two groups: the control group(dropping with PBS) and the HA treated group(dropping with 10mg/ml HA). The healing rate of the epithelial defect area as well as the
morphologic
changes were observed, and the healing response of the cornea was analyzed immunohistochemically to determine the distribution of fibronectin.
@ES The results were as follows;
@EN 1. The reduction of the epithelial defect area after 15hrs following laser keratectomy was significantly accelerated in the HA treated group compared with the control group(P<0.005). The mean epithelial healing rate was 0.38±0.05mm*/hr in
the
HA
treated group and 0.32±0.05mm*/hr in the control group.
2. Histologically, remarkable epithelial thickening and less polymorphonuclear leukocyte infiltration under leading epithelium in the HA treated group were observed. of which findings were limited in anteior stroma.
3. Under transmission electron microscope, enlargement of the intercellular space of leading epithelium was prominent and incomplete lamina densa was shown in the control group, whereas some hemidesmosomes in HA treated group. After 72 hrs, the
intercellular spaces of wing cell layer were bridged by desmosomes more tightly, and remarkable hemidesmosomes were shown in HA treated group compared with the control group.
4. Under scanning electron microscope at 72 hrs following keratectomy, the wound area was covered with epithelium with well developed linear microvilli in both groups.
5. Fibronectin immunoreactivity was observed under the leading epithelium using immunohistochemistry at 15 hrs following keratectomy in both groups.
The results indicate that hyaluronic acid stimulates epithelial wound healing following excimer laser keratectomy by enhancing cell migration and promoting the tight intercellular junction. The expression of fibronectin was not influenced by HA.
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